Dermatology

Artificial Intelligence–Detected Tumor-Infiltrating Lymphocytes and Outcomes in Anti–PD-1–Based Treated Melanoma

JAMA Oncology

JAMA Oncology

Research Authors: Mark Schuiveling, MD; Isabella A. J. van Duin, PhD; Laurens S. ter Maat, PhD; Janneke C. van der Weerd, MD; Rik J. Verheijden, MD; Franchette van den Berkmortel, PhD; Christian U. Blank, PhD; Gerben E. Breimer, PhD; Femke H. Burgers, MD; Marye Boers-Sonderen, PhD; Alfons J. M. van den Eertwegh, PhD; Jan Willem B. de Groot, PhD; John B. A. G. Haanen, PhD; Geke A. P. Hospers, PhD; Ellen Kapiteijn, PhD; Djura Piersma, PhD; Gerard Vreugdenhil, PhD; Hans Westgeest, PhD; Anne M. R. Schrader, PhD; Josien P. W. Pluim, PhD; Paul J. van Diest, PhD; Mitko Veta, PhD; Karijn P. M. Suijkerbuijk, PhD; Willeke A. M. Blokx, PhD

Research Authors: Mark Schuiveling, MD; Isabella A. J. van Duin, PhD; Laurens S. ter Maat, PhD; Janneke C. van der Weerd, MD; Rik J. Verheijden, MD; Franchette van den Berkmortel, PhD; Christian U. Blank, PhD; Gerben E. Breimer, PhD; Femke H. Burgers, MD; Marye Boers-Sonderen, PhD; Alfons J. M. van den Eertwegh, PhD; Jan Willem B. de Groot, PhD; John B. A. G. Haanen, PhD; Geke A. P. Hospers, PhD; Ellen Kapiteijn, PhD; Djura Piersma, PhD; Gerard Vreugdenhil, PhD; Hans Westgeest, PhD; Anne M. R. Schrader, PhD; Josien P. W. Pluim, PhD; Paul J. van Diest, PhD; Mitko Veta, PhD; Karijn P. M. Suijkerbuijk, PhD; Willeke A. M. Blokx, PhD

AIIM Authors: Hanna Zhu, Josh Bronte

AIIM Authors: Hanna Zhu, Josh Bronte

Publication Date: Oct 16, 2025

Publication Date: Oct 16, 2025

Comprehensive Summary

This study investigates whether AI-based detection of tumor-infiltrating lymphocytes (AI-TILs) can predict treatment outcomes in patients with metastatic melanoma receiving first-line anti-PD-1 immunotherapy. The researchers applied an open source AI model (Hover-NeXt) to determine the percentage of lymphocytes on standard H&E slides, using pretreatment metastatic tumor samples from 1202 patients across multiple Dutch centers. The researchers compared AI-TIL levels with objective response, progression-free survival, and OS. The results indicated that higher AI-TIL percentages were associated with improved treatment response and longer PFS and OS. Compared to manually scored TIL, AI-TILs showed stronger correlations with outcomes, proving their potential as a biomarker. However, the authors note that AI-TILs could complement, but not replace existing pathology assessments.

Outcomes and Implications

AI-based analysis of tumor immune infiltration could become a practical and less costly tool to guide melanoma treatment decisions. Counting TILs could help clinicians identify which patients would benefit from anti-PD-1 therapy and could help in determining personalized treatments plans. This approach could improve prognostic accuracy and reduce unnecessary combination therapies for the future.

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